Contact an Account ManagerChromatography of bis-quaternary amino steroids II. Separation on chemically bonded silica phases
Summary
Type: HPLC
Application #: 109390
Catalog#: 720024.46 - MN HPLC Column, Nucleosil CN, 100A, 10um, 4.6x250mm
Detection: UV, 218nm
Conditions: eluent: organic phase - ammonia solution (86:14), organic phase = methanol - acetonitrile in different ratios, CH3OH content in organic phase A) 0%, B) 12.8%, C) 25.6%, D) 50%, E) 74.4%, F) 87.2%, G) 100%
flow rate 1ml/min
Authors: Gazdag M
Source: J Chromatogr 347 (1985) 284 - 290
Details
Legend: Fig. 5. Dependence of the separation on the ratio of methanol to acetonitrile.